Effects of Age on Fasting Blood Glucose, Liver and Muscle Glycogen Levels in the Common African Toad Bufo regularis
Aging is associated with elevated blood glucose levels, insulin resistance and pancreatic islet dysfunction. The age-related dysfunction of glucose metabolism has been well studied in humans and rodents. However, there is paucity of information on ageing and glucose metabolism in amphibians. This study was designed to investigate the effect of ageing on fasting blood glucose, liver and muscle glycogen levels in the common African toad, Bufo regularis. Toads of different sizes were randomly picked and used for the study. They were fasted for 24hours and anaesthetized with sodium thiopentone (50mg/kg) given intraperitoneally. Blood samples were collected from truncus arteriosus for blood glucose determination while the liver and muscle glycogen levels were determined using anthrone reagents methods. Ages of toads were determined using modified skelectochronology technique. The fasting glucose level increased with advancing age while liver and muscle glycogen levels decreased with age in Bufo regularis. The results suggest that glucose metabolism slows down with age in the toads and demonstrated similarities between the toads, humans and rodents in relation to age-related changes in glucose and glycogen metabolism
Broughton, D. L., R. Taylor. 1991. Review: deterioration of glucose tolerance with age: the role of insulin resistance. Age Ageing 20:221-5 Medline: 1853796
Cartee, G. D., D. A. Young., M. D. Sleeper, J. Zierath., H. Wallberg-Henriksson, and J. O. Holloszy. 1989. Pro- longed increase in insulin-stimulated glucose transport in muscle after exercise. Am. J. Physiol. 256: E494– E499.
Castanet, J. and F. Smirina. 1990. Introduction to skeletochronological method in amphibians and reptile. Annales Des science Naturelles Comprenant la Zoologie; 13:191-196
Change, A. M, and J.B. Halter. 2003. Aging and insulin secretion Am. J. Physiol. Endorcinol Meta. 284 E7 -12 medline: 12485807.
Defronzo, R. A. 1981. Glucose Intolerance and aging. Diabetes care 4:493-501 (Pub Med: 7049632).
Ferrannini, E, A. Natali., B. Capaldo., M. Lehtovirta., S. Jacob., H. V. Yki-Jar. 1996. Insulin resistance, hyperinsulinemia, and blood pressure: role of age and obesity. European group for the study of insulin resistance, (EGIR). Diabetes 45: 947-53 Medline: 8666147.
Gu, Z., Y. Du., C. Li., Y. Liu, L. Ma., L. Li, Y. Gong., H. Tiam., and C. Li. 2012. Effect of aging on islet beta cell function and its mechanisms in Wistar rats Age: 34: 1393 – 1403.
Houmard, J. A., M. D. Weidner., P. L. Doland., N. Leggett- Frazier.,K. E. Gavigan., M.S. Hickey, et al. 1995. Skeletal muscle Glucose protein concentration and aging in humans. Diabetes 44:555-60, DOI: 10, 2337/Diabetes, 44.5.555.
Isehunwa, G.O., O. T. Olaniyan., and A. R. A. Alada. 2013. The role of alpha and beta adrenergic receptors in cortisol-induced hyperglycemia in the common African toad Bufo regularis. Afri. J. Biotech. 12:(36).5554 – 5558.
Ivey, P. A., G. A. Gaesser. 1987. Postexercise muscle and liver glycogen metabolism in male and female rat. J. Appl. Physiol. 62:1250-1254
Jackson, A R. 1990. Mechanism of Age-Related Glucose Intolerance. Diabetes care; 13(Suppl. 2):9-19
Jermyn, M. A, (1975). Increasing the sensitivity of the anthrone method for carbohydrate. Anal. Biochem. 68:322-335
Kalyani, R. R. and J. M. Egan. 2013. Diabetes and Altered glucose metabolism with aging. Endocrinol. Metab. 42(2): 333-347.
Khan S. H., U. Mashood., M. S. Hanif., S. O. R. S. Bokhari., M. J. Khan. 2012. Effect of age and gender on blood lipids and glucose. Rawal Med. J. 37 (4): 344- 347.
Khandelwal, R. L., T. L. Enno., N. Narayanan. 1989. Effect of age on glycogen synthase and phosphorylase activities in rat liver. Mach Ageing Dev. 28(1):13-22
Khonsue, W., M. Matsui., and Y. Misawa, Y. 2000. Age determination by skeletochronology of Rana Nigrovittatat, a frog from tropical forest of Thailand. Zool. Sci. 17:253-257.
Ko, G. T. C., H. P. S. Wal., J. S. F. Tang. 2006. Effect of age on plasma glucose levels in non-diabetic Hong Kong Chinese. Croat. Med. J. 47: 709-13.
Kollberg, G., M. Tulinius., T. Gilljam., I. Ostman-Smith., G. Forsander., P. Jotorp., A. Oldfors., E. Holme. 2007. Cardiomyopathy and exercise intolerance in muscle glycogen storage disease. New Eng. J. Med. 357:1507–1514. [PubMed: 17928598]
Kumber, S. M. and K. Pancharatna. 2004. Annual formation of growth marker in a tropical amphibian. Herpetol. Rev. 35:35-37.
Kutty, V. R., C. R. Soman., A. Joseph., K. V. Kumar., and R. Pisharody. 2002. Random capillary blood sugar and coronary risk factors in a South Kerela population. J. Cardio. Risk. 9: 361-7 Medline: 12478206.
Lee, Z. S., J. C. Chan., V. T. Yeung, C .C. Chow., M. S. Lau., G. T. Ko. et al.1999. Plasma insulin, growth hormone, cortisol and central obesity among young Chinese type 2 diabetic patients. Diabetes Care 22:1450-7. Medline; 10480508.
Lucia, A., G. Nogales-Gadea., M. Pérez., M. A. Martín., A. L. Andreu, J. Arenas. 2008. McArdle disease: what do neurologists need to know? Nat Clin. Pract. Neurol. 2008; 4:568–577. [PubMed: 18833216]
Manchester, J., A. V. Skurat., P. Roach., S. D. Hauschka., J. C. Jr. Lawrence. 1996. Increased glycogen accumulation in transgenic mice overexpressing glycogen synthase in skeletal muscle. Proc. Natl Acad. Sci. USA. 93:10707–10711. [PubMed: 8855244]
Montori-Grau, M., R. Minor., C. Lerin., J. Allard., C. Garcia-Martinez., R. de-Cabo, and A. M. Gomez-Foix. 2009. Effects of aging and calorie restriction on rat skeletal muscle glycogen synthase and glycogen phosphorylase. Exp. Gerontol : 44(6-7): 426 – 433.
Pederson, B. A., H. Chen., J. M. Schroeder., W. Shou., A. A. DePaoli-Roach., P. J. Roach. 2004. Abnormal cardiac development in the absence of heart glycogen. Mol. Cell Biol. 2004; 24:7179–7187. [PubMed: 15282316]
Richter, E. A., L. P. Garetto., M. N. Goodman., N. B. Ruderman. 1982. Muscle glucose metabolism following exercise in the rat: increased sensitivity to insulin. J. Clin. Invest. 69:785–793. [PubMed: 6804492]
Seifter, S., S. Dayton., B. Novic., E. Muntwryler. 1950. Isolation and purification. The estimation of glycogen with anthrone reagent. Archiv. Biochem. 25: 191-199
Stout, R. W. 1994. Glucose tolerance and ageing. J. R. Soc. Med. 87: 608-9 Medline: 7966111
Suji, G., K. S. Siva. 2004. Glucose, glycation and aging. Biogerontology 5:365-73, DOI: 10.1007/5 10522-004-3189-0.
Trinder, E. 1969. Determination of blood glucose using 4 amino phenazone as oxygen acceptor. J. Chem. Pathol. 22:246-248.
Wu, M., D. M. Desai., S. K. Kakarla., A. Katta., S. Paturi., A. K. Gutta., K. M. Rice., E. M. Walker., and E. R. Blough, 2009. A Cetamoniphen prevents aging – associated hyperglycernia in aged rats: Effect of aging – associated hyper-activation of p38 – MAPK and ERK1/2. Diabetes Metab. Res. Rev, 25: 279 – 286 doi: 10.1002/dmrr. 932.
Yue F., G. Zhang., R. Tang., Z. Zhang., L. Teng., Z. Zhang. 2016. Age and sex-related changes infanting plasma glucose and Lipo protein in Cynomolgus Monkeys lipids in Health and Disease. 15:111-121