Piptandenia Africana and Nauclea latifolia protects against Diethylnitrosamine induced hepatic tumors in Albino Wistar rats

  • Solomon E. Owumi
  • Sarah O Nwozo
  • Queen-Esther Akinade
  • Olubunmi A. Akinwumi

Abstract

Piptandenia africana (PA) and Nauclea latifolia (NL) showed anti-proliferative effects on Hela, MCF7 and ICMST-6 cells. We examined the effect of NL and PA as phytotherapeutic candidates against N-nitrosodiethylamine (DEN)-induced hepatic toxicities and tumorigenesis.

Wistar’s rat were treated as follows: (1)Control, (2)DEN, (3)PA, (4)NL,  (5)PA+NL, (6)DEN+ PA, (7)DEN+ NL, (8)DEN+PA+NL. Hepatic transaminases were assessed in serum for hepatoxicity, in addition to bilirubin, blood glucose levels and hepatic antioxidants status. Furthermore, DEN-induced tumors were estimated, and sectioned for histopathological examination.

Animals treated with DEN only, had mean tumor of five (T5), these were reduced to T2, T2 and T1.75 in groups VI, VII and VIII respectively. Blood glucose levels in DEN treated rats were lowest (87.5 ± 5.1) compared to other treated rats.

Elevated markers of oxidative stress and lower antioxidant profile, suggests that group 2 animals were stressed oxidatively compared to other groups. Significantly higher markers of hepatic toxicity indicated DEN injuriousness was highest in DEN only treated animals. Limited histopathology indicated severe mononuclear cells infiltration of the portal triad, and multiple foci of hepatic necrosis in group 2 animals, compared to control. PA, SL and in combination treated animals were devoid of these pathological hallmarks emblematic in DEN treated animals.

Corroborating In vitro findings, treatment with PA and NL mitigated DEN-induced hepatic tumorigenesis. This phytoprotective capacity unveiled, maybe pertinent in averting hepatic damage from potential chemical carcinogens. Elucidating the phytochemistry and mechanisms of PA and NL mode of action is necessary, which could serve as potential anti-tumor drug candidate

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Published
2019-03-12
Section
Carcinogenesis/Oncology/Chemoprevention/Toxicology/Biomarkers/Oxidative Stress