Alterations in Reproductive Indices in Mice Exposed to Contaminated Urban Groundwater From An Automobile Spare Parts Market in Ibadan, South-Western Nigeria

  • Adeola Anike Oni Department of Zoology, University of Ibadan, Ibadan
  • Obokparo Ohore Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan.
  • Ijeoma Amama Department of Zoology, Faculty of Science, University of Ibadan, Ibadan.

Abstract

Contamination of underground water with pollutants such as spent engine oil from automobile scrap sales and repair activities has health implications on biota and public health. This study investigated the effects of contaminated groundwater within the Araromi spare-parts market, Ibadan on some reproductive indices in male Swiss albino mice. Samples from two most contaminated wells (CWW1 and CWW7) out of seven well-water bodies were purposively selected for experimental studies based on high levels of heavy metals or PAHs concentrations respectively. Four groups of 10 mice each were exposed to graded concentrations (25%, 50%, 75%, and 100%) of the well-water samples, while a control group of 10 mice received distilled water. Semen characteristics, reproductive hormonal levels (LH and FSH) were analysed at days 21, 42 and 84 post-exposure for changes induced by the contaminated well-water. Data were analysed using one-way ANOVA. Elevated levels of carcinogenic PAHs (0.01±0.01 - 0.62±0.42ug/L) and Pb (0.20±0.21mg/L) were recorded in the contaminated wells. Relative water intake in the control group was significantly (p<0.05) higher than in treatment groups except for 25% and 100% CWW1 at day 84; and 100% CWW7 at day 42. Sperm count and percentage sperm motility were significantly lower (p<0.05) in exposed mice compared to the control and lower concentration groups (25%) throughout the experiment. Various types of morphological abnormalities including long pin and pin head were observed at day 84 in 75% CWW1 and 100% CWW7 respectively. Significantly elevated (p<0.05) levels of LH and FSH occurred in 100% CWW1 at day 84, while FSH levels were only significantly higher in 100% CWW7 exposed group at day 84. Our findings indicate that the contaminated well-water induced reproductive toxicity in exposed mice and may have negative health implications in orally exposed human populations with prolonged domestic usage of the contaminated water.

 

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Published
2019-10-31
Section
Carcinogenesis/Oncology/Chemoprevention/Toxicology/Biomarkers/Oxidative Stress