Human Respiratory Syncytial Virus (HRSV) A and B infection among children attending primary and secondary health facilities in Ibadan, Nigeria
Human respiratory syncytial virus (HRSV) causes high morbidity and mortality in infants and young children. There is high prevalence of the virus in Nigeria, however the circulating subtype is not known. We report here the subtypes of HRSV circulating among children in Ibadan. Two hundred and thirty-one nasopharyngeal and oropharyngeal swabs were collected from children presenting with respiratory infections in Secondary Health Facility (SHF) for care as well as those attending immunization centers in Primary Health Centers (PHCs) in Ibadan, Nigeria. Viral RNA was extracted directly from the clinical specimen and used for HRSV detection with a pair of primers that targets the conserved region of the viral matrix gene. HRSV-positive samples were subtyped using subtype-specific primers. The prevalence of HRSV infection was 8.7% and 34.6% in PHCs and SHF respectively. Both subtypes of HRSV were detected co-circulating in PHCs and SHF. No evidence of coinfection of HRSV A and B was found. HRSV-A was the predominant subtype detected in SHF while subtype B was predominant among participants recruited at PHCs. Higher disease severity scores were associated with HRSV – A infection than infection with HRSV – B. All the HRSV detected from those diagnosed of bronchopneumonia and bronchiolitis were subtype A. In conclusion, subtypes A and B were co-circulating in PHCs and SHF in Ibadan, with HRSV – A being the predominant. Additional study with samples collected across both wet and dry seasons and from other parts of the country will be done to determine the pattern of HRSV circulation in Nigeria
Akinloye, O. M., Rönkkö, E., Savolainen-Kopra, C., Ziegler, T., Iwalokun, B. A., Deji-Agboola, M. A., … Hovi, T. (2011). Specific viruses detected in Nigerian children in association with acute respiratory disease. Journal of Tropical Medicine, 2011, 1–6. http://doi.org/10.1155/2011/690286
Beem, M. (1967). Repeated infections with respiratory syncytial virus. J Immunol., 98(6), 1115–22.
Bigogo, G. M., Breiman, R. F., Feikin, D. R., Audi, A. O., Aura, B., Cosmas, L., … Burton, D. C. (2013). Epidemiology of respiratory syncytial virus infection in rural and urban Kenya. Journal of Infectious Diseases, 208(SUPPL. 3), 207–216. http://doi.org/10.1093/infdis/jit489
D´Elia, C., Siqueira, M., Portes, S., & Sant’Anna, C. (2005). Respiratory syncytial vírus – associated lower respiratory tract infections in hospitalized infants. Rev Soc Bras Med Trop., 38, 7–10.
Dong, L., Dai, L., Fan, J., Chen, X., Jin, X., Zhang, Y., & Lin, H. (2015). Epidemiologic characteristics and the relationship with disease severity of respiratory syncytial virus genotypes from children with lower respiratory tract infection in the southern Zhejiang province. Chin J Pediatr, 53(7), 537–541.
Eshaghi, A., Duvvuri, V. R., Lai, R., Nadarajah, J. T., Li, A., Patel, S. N., … Gubbay, J. B. (2012). Genetic variability of human respiratory syncytial virus A strains circulating in Ontario: a novel genotype with a 72 nucleotide G gene duplication. PloS One, 7(3), e32807. http://doi.org/10.1371/journal.pone.0032807
Fall, A., Dia, N., Cisse, E. H. A. K., Kiori, D. E., Sarr, F. D., Sy, S., … Niang, M. N. (2016). Epidemiology and molecular characterization of human respiratory syncytial virus in Senegal after four consecutive years of surveillance, 2012-2015. PLoS ONE, 11(6), 1–15. http://doi.org/10.1371/journal.pone.0157163
Falsey, A. R., Hennessey, P. A., Formica, M. A., Cox, C., & Walsh, E. E. (2005). Respiratory syncytial virus infection in elderly and high-risk adults. The New England Journal of Medicine, 352(17), 1749–59. http://doi.org/10.1056/NEJMoa043951
Faneye, A., Motayo, B. O., Adesanmi, A., & Onoja, B. (2014). Evaluation of IgG Antibodies Against Respiratory Syncytial Virus (RSV), and Associated Risk Factors for Severe Respiratory Tract Infections in Pre-School Children in North-Central, Nigeria. African Journal of Infectious Diseases, 8(2), 36–39.
Glezen, P., & Denny, F. (1973). Epidemiology of acute lower respiratory disease in children. N Engl J Med., 288(10), 498–505. http://doi.org/10.1056/NEJM197303082881005
Global Burden of Diseases and Injuries in Children and Adolescents. (2017). Global and National Burden of Diseases and Injuries Among Children and Adolescents Between 1990 and 2013 Findings from the Global Burden of Disease 2013 Study. JAMA Pediatr. 2016;170(3):267-287., 98121(3), 267–287. http://doi.org/10.1001/jamapediatrics.2015.4276
Human, S., & Moore, M. L. (2016). How close are we to a respiratory syncytial virus vaccine? Future Virol.
Johnson, B., Osinusi, K., Aderele, W., & Tomori, O. (1993). Viral pathogens of acute lower respiratory infections in pre-school Nigerian children and clinical implications of multiple microbial identifications. West African Journal of Medicine, 12(1), 11–20.
Lamarao, L. M., Ramos, F. L., Alencar de Mello, W., Santos, M. C., Barbagelata, L. S., Justino, M. C. A., … Linhares, A. D. C. (2012). Prevalence and clinical features of respiratory syncytial virus in children hospitalized for community-acquired pneumonia in northern Brazil. BMC Infectious Diseases, 12(1), 119. http://doi.org/10.1186/1471-2334-12-119
Lee, J., Chang, L., Wang, L., Kao, C., Shao, P., Lu, C., … Huang, L. (2007). Epidemiology of respiratory syncytial virus infection in northern Taiwan, 2001–2005 – seasonality, clinical characteristics, and disease burden. J Microbiol Immunol Infect., 40, 293–301.
Malasao, R., Okamoto, M., Chaimongkol, N., Imamura, T., Tohma, K., Dapat, I., … Oshitani, H. (2015). Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines. PloS One, 10(11), 2012–2013. http://doi.org/10.1371/journal.pone.0142192
McConnochie, K., Hall, C., Walsh, E., & Roghmann, K. (1990). Variation in severity of respiratory syncytial virus infections with subtype. J Pediatr., 117(1), 52–62.
Midulla, F., Scagnolari, C., Bonci, E., Pierangeli, A, Antonelli, G., De Angelis, D., Berardi, R & Moretti, C. (2010). Respiratory syncytial virus, human bocavirus and rhinovirus bronchiolitis in infants. Archives of Disease in Childhood, 95(1), 35–41. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19822538
Mlinaric-galinovic, G., Tabain, I., Kukovec, T., Vojnovic, G., Bozikov, J., Bogovic-cepin, J., … Welliver, R. C. (2012). Analysis of biennial outbreak pattern of respiratory syncytial virus according to subtype (A and B) in the Zagreb region. Pediatr Int., 54(3), 331–335. http://doi.org/10.1111/j.1442-200X.2011.03557.x
Mlinaric-galinovic, G., Vojnovic, G., Cepin-bogovic, J., Bace, A., Bozikov, J., Welliver, R. C., … Cebalo, L. (2009). Does the viral subtype influence the biennial cycle of respiratory syncytial virus? Virology Journal, 6(133), 1–7. http://doi.org/10.1186/1743-422X-6-133
Mufson, M., Belshe, R., Orvell, C., & Norrby, E. (1988). Respiratory syncytial virus epidemics: variable dominance of subgroups A and B strains among children, 1981-1986. J Infect Dis, 157(1), 143–148.
Nair, H., Nokes, D. J., Gessner, B. D., Dherani, M., Madhi, S. A., Singleton, R. J., … Campbell, H. (2010). Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systemic review and meta-analysis. Lancet, 375(9725), 1545–1555. http://doi.org/doi: 10.1016/S0140-6736(10)60206-1
Nwankwo, M., Dym, A., Schuit, K., Offor, E., & Omene, J. (1988). Seasonal variation in respiratory syncytial virus infections in children in Benin-City, Nigeria. Trop Geogr Med. 40(4): 40(4), 309–13.
Odaibo, G. N., Omotade, O. O., Forbi, J. C., & Olaleye, D. O. (2013). Incidence and Burden Of RSV Infection in A Community-Based Cohort of Under-Five Years Children In Nigeria. Archives of Basic and Applied Medicine, 1(1), 67–72.
Okiro, E. A., Ngama, M., Bett, A., & Nokes, D. J. (2012). The Incidence and Clinical Burden of Respiratory Syncytial Virus Disease Identified through Hospital Outpatient Presentations in Kenyan Children. PLoS ONE, 7(12), 1–7. http://doi.org/10.1371/journal.pone.0052520
Olaleye, O., Olawuyi, O., & Baba, S. (1992). Sero-epedimiological studies of respiratory syncytial and adeno viruses in children in Ibadan, Nigeria, 1985 – 1988. Trans R Soc Trop Med Hyg., 86(3), 294–7.
Peret, T. C. T., Hall, C. B., Schnabel, K. C., Golub, J. A., & Anderson, L. J. (1998). Circulation patterns of genetically distinct group A and B strains of human respiratory syncytial virus in a community, 79(9), 2221–2229.
Piedimonte, G., & Perez, M. K. (2014). Respiratory syncytial virus infection and bronchiolitis. Pediatrics in Review / American Academy of Pediatrics, 35(12), 519–30. http://doi.org/10.1542/pir.35-12-519
Robertson, S. E., Roca, A., Alonso, P., Simoes, E. A. F., Kartasasmita, C. B., Olaleye, D. O., … Wright, P. W. (2004). Respiratory syncytial virus infection: denominator-based studies in Indonesia, Mozambique, Nigeria and South Africa. Bulletin of the World Health Organization, 82(12), 914 – 922.
Scott, P. D., Ochola, R., Ngama, M., Okiro, E. A., Nokes, D. J., Medley, G. F., & Cane, P. A. (2004). Molecular Epidemiology of Respiratory Syncytial Virus in Kilifi District, Kenya. J Med Virol., 74, 344–354. http://doi.org/10.1002/jmv.20183
Scott, P., Ochola, R., Sande, C., Ngama, M., Okiro, E., Medley, G., … Cane, P. (2007). Comparison of strain-specific antibody responses during primary and secondary infections with respiratory syncytial virus. J Med Virol., 79(12), 1943–50. http://doi.org/10.1002/jmv.20999
Shay, D. K., Holman, R. C., Newman, R. D., Liu, L. L., Stout, J. W., & Anderson, L. J. (1999). Bronchiolitis-associated hospitalizations among US children, 1980-1996. JAMA : The Journal of the American Medical Association, 282(15), 1440–1446.
Sricharoenchai, S., Palla, E., Pasini, F. L., & Sanicas, M. (2016). Epidemiology of Respiratory Syncytial Virus Lower Respiratory Tract Infection (RSV-LRTI) In Children in Developing Countries. Journal of Tropical Diseases and Public Health, 4(3), 4–11. http://doi.org/10.4172/2329-891X.1000212
Taylor, C., Morrow, S., Scott, M., Young, B., & Toms, G. (1989). Comparative virulence of respiratory syncytial virus subgroups A and B. Lancet, 1(8641), 777–778.
Tran, D. N., Pham, T. M. H., Ha, M. T., Tran, T. T. L., Dang, T. K. H., Yoshida, L.-M., … Ushijima, H. (2013). Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. PloS One, 8(1), e45436. http://doi.org/10.1371/journal.pone.0045436
Trento, A., Viegas, M., Galiano, M., Carballal, G., Mistchenko, A. S., Melero, A., & Videla, C. (2006). Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the. Journal of Virology, 80(2), 975–984. http://doi.org/10.1128/JVI.80.2.975
Walsh, E., McConnochie, K., Long, C., & Hall, C. (1997). Severity of respiratory syncytial virus infection is related to virus strain. J Infect Dis, 175(4), 814–820.
Weber, M. W., Mulholland, E. K., & Greenwood, B. M. (1998). Respiratory syncytial virus infection in tropical and developing countries. Tropical Medicine and International Health, 3(4), 268 – 280. http://doi.org/10.1046/j.1365-3156.1998.00213.x