Isolation, Characterization and Identification of Plant Growth Promoting Rhizobacteria from Cauliflower (Brassica oleracea)

  • Fazal Mehmood Khan
  • Haq Inamul
  • Sultan Tariq
  • Farooq Muhammad
  • Chinenyenwa M.D Ohia
  • Ahmad Tauseef

Abstract

Bio-fertilizers are currently being considered as effective and environmentally-safe alternatives to synthetic fertilizers. In recent years, the use of many Rhizobia in the growth of many plants has found commercial utilization in developing countries especially as growth promoters. This study was conducted to isolate Rhizobacteria from the rhizosphere, rhizoplane and endorhizosphere of cauliflower (Brasicca oleracea) collected from ChattaBakhtawar, Islamabad, Pakistan. Luria-Bertini (LB) media was used to isolate rhizobacterial strains. The identification of rhizobacteria was done by studying many morphological characteristics of colonies growing on the media. These characteristics include Form, Elevation, Margin, Opacity and Colour; these were recorded periodically. For the confirmation of rhizobacteria; biochemical tests were performed. Gram staining was done for 14 strains CF-ES-4, CF-ES-5A, CF-ES-5B, CF-ES-5C, CF-ES-6A, CF-ES-6B, CF-RP-4C, CF-RP-4B, CF-RP-6, CF-RP-5A, CF-RP-4, CF-RS -4, CF-RS-5, and CF-RS-6: Among these, 11 strains were Gram negative while the 3 strains CF-RP-5A, CF-ES-5A and CF-RP-4 were Gram positive. The strains which were phosphate negative  CF-RP-4C, CF-RS-8, CF-RP-4A, CF-ES-4, CF-RS-7, CF-ES-5C, CF-RP-6 while the CF-RP-5A CF-RP-5B, CF-ES-5B, CF-ES-5A, CF-RP-4B, CF-ES-6B, CF-RS-4 and CF-RS-6 strains were phosphate positive; these confirmed qualities of rhizobacteria. Extraction of rhizobacteria was also done and the composition was maintained accordingly as for biofertilizers. Taken together, the present investigation adds more information to current understanding of the mechanism of Nitrogen-fixing by rhizobacteria and will be useful for the commercialization of these strains to improve the yield of economic plants. Thus, isolation of Rhizobia and its use in agricultural field practice should be adopted.

References

1. Bais, H.P., Fall, R., and Vivanco, J.M. 2004. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringaeis facilitated by biofilm formation and surfactin production. Plant Physiol. 134, 307–319.doi: 10.1104/pp.103.028712
2. Bargabus, R.L., Zidack, N.K. Sherwood, J.E. and Jacobsen, B.J. 2002. Characterization of systemic resistance in sugar beet elicited by a non- pathogenic, phylosphere-colonizing Bacillus mycoides, biological control agents. Physiol. Mol. Plant Pathol. 61, 289–298. doi:10.1006/pmpp.2003.0443
3. Cattelan, A.J., Hartel, P.G. and Fuhrmann, J.J. 1999. Screening for plant growth- promoting rhizobacteria to promote early soybean growth. Soil Sci. Soc. Am. J. 63, 1670–1680. doi:10.2136/sssaj1999.6361670x
4. Chen,X.H.,Koumoutsi,A.,Scholz,R.,Schneider,K.,Vater,J.,Süssmuth,R., et al. 2009. Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens. J. Biotechnol. 140, 27–37. doi: 10.1016/j.jbiotec.2008.10.011
5. El-Sayed, S.W., Akhkha, A., El-Naggar, M.Y. and Elbadry, M. 2014. In vitro antagonistic activity, plant growth promoting traits and phylogenetic affiliation of rhizobacteria associated with wild plants grown in arid soil. Front. Microbiol. 5:651. doi:10.3389/fmicb.2014.00651
6. Gerhardson, B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20, 338–343. doi:10.1016/S0167-7799(02)02021-8
7. Kloepper, J.W., Leong, J., Teintze, M. and Schroth, M.N. 1980. Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature 268, 885–886. doi:10.1038/286885a0
8. Liu, C.H., Chen, X., Liu, T.T., Lian, B., Gu,Y., Caer,V., et al. 2007. Study of the antifungal activity of Acinetobacter baumannii LCH001 in vitro and identification of its antifungal components. Appl. Microbiol. Biotechnol. 76, 459–466. doi:10.1007/s00253-007-1010-0
9. Mudili J. 2007. Introductory Practical Microbiology. Oxford: Alpha Science International Ltd.
10. Pikovskaya R.I. 1948. Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologiya 17 : 362–370.
11. Turan, M., Ataoglu N., and Sahin F. 2007. Effects of Bacillus FS-3 on growth of tomato (Lycopersicon esculentum L.) plants and availability of phosphorus in soil. Plant and Soil Environment 53:58-64.
12. Woyessa, D. and Assefa, F. 2011. Effects of Plant Growth Promoting Rhizobaceria on Growth and Yield of Tef (Eragrostistef Zucc.Trotter) under Greenhouse Condition. Research Jour. of Microbio..6: 343-355.
Published
2018-05-02
Section
Section B (Basic Science Research)