Assessment of cytotoxicity of leaf extracts of Andrographis paniculata and Aspilia africana on murine cells in vitro

  • Adeola A Ala
  • B B Olotu
  • C.M.D Ohia

Abstract

People, especially in rural environments and recently, those dissatisfied with conventional medicine use medicinal plants for their therapeutic effects. Two or more plants are usually combined for such use and these combinations cause interactions that may be safe but could also be detrimental to health. Used singly, Andrographis paniculata and Aspilia africana are considered safe plants with low toxicities but little is known about their safety in combined therapy. This study assessed the cytotoxic activity of the aqueous, methanol and chloroform extracts of the two plants in a 1:1 combination at various concentrations on murine hepatocytes, thymocytes and splenocytes. The murine cells were seeded in microtitre plates and tested with the combined extracts at different concentrations and percentage viability values of the cells determined by the blue tetrazolium reduction assay (MTT). The extracts used were: combined aqueous extract of the two plants (CAE), and combined chloroform extract (CCE). At 72hr incubation with 500 μg/ml extract concentration, percentage viability was low. Splenocytes thymocytes and hepatocytes incubated with CAE had viability values of 35%, 28% and 64% respectively and with CCE, 26%, 26% and 36% respectively. From the results, the extracts showed no protective effect on the murine cells at this concentration and incubation period. The low values indicate cytotoxic interactions in the combined extracts. However more research is needed to understand the  biochemical pathways involved

References

Agonihotri S., Wakode, S and Agonihotri, A. 2010. An overview of anti-inflammatory properties and chemo-profiles of plants used in traditional medicine. Indian Journal of Natural Products and Resources 1 (2): 150-167.
Akuodor G. C., Amos, G. M., Essien, A. D., Essien, D.O., Akpan, J. L., Ezeokpo, B. C. 2012. Antimalarial potency of the leaf extract of Aspilia africana (Pers.) C.D. Adams. Asian Pacific Journal of Tropical Medicine 126-129
Bhattacharya Somshuvra and Das Binay 2012. Anti-diabetic activity of Lagenaria siceraria pulp and seed extract in normal and alloxan-induced diabetic rats. IJPSR 3(9): 3362-3369 ISSN: 0975-8232
Da Silva A. S, Tonin A. A, Thorstenberg M. L, Leal D. B, Fighera R, Flores M. M, França R. T, Camillo G, Vogel F. S, de la Rue M, Lopes S. T. 2013. Relationship between butyrylcholinesterase activity and liver injury in mice acute infected with Toxoplasma gondii. Pathol Res Pract. 15; 209 (2):95-8.
Farnsworth, N. R., Akerele, O., Bingel, A. S., Soejarto, D. D., Guo, Z., 1985.Medical plants in therapy. B. World Health Organ. 63, 965-981.
Hoyos, L.S., Au, W.W., Heo, M.Y., Morris, D.L.andLegator, M.S. (1992). Evaluation of the genotoxic effects of a folk medicine, Petiveria alliacea (anamu). Mutation Research, 280, 29- 34
Iwu M. M. 1993. Handbook of African Medicinal Plants, CRP Press Boca Raton, Florida, p88.
Manosroi, A.; Saraphanchotiwitthaya, A.; Manosroi, J. 2003. Immunomodulatory activities of Clausena excavate Burm. f. wood extracts. J. Ethnopharmacol., v.89, n.1, p.155-160.
Mehrotra, S.; Mishra, K. P.; Maurya, R.; Srimal, R.C.; Yadav, V. S.; Pandey, R.; Singh, V. K. 2003. Anticellular and immunosuppressive properties of ethanolic extract of Acoruscalamus rhizome. Int. Immunopharmacol., v.3, n.1, p.53-61.
Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, v.65, n.1-2, p.55-63.
Oko, O.O.K., Agiang, E.A., Osim, E.E. and Asuquo, O.R. (2011). Toxicological Evaluation of Aspilia Africana Leaf Extracts in mice. American Journal of Pharmacology and Toxicology 6 (3): 96-101.
Okoli, C.O., Akah, P.A. and Okoli, A.S. 2007a. Potentials of leaves of Aspiliaafricana (Compositae) in wound care: an experimental evaluation. Complementary and Alternative Medicine Research. Vol 7:24. http://www.biomedcentral.com/1472-6882/7/24
Okoli C. O., Akah P. A., Nwafor S. V., Anisiobi A. J. Ibegbunam I. N., Erojikwe O. 2007b. Antinflammatory Activity of Hexane leaf Extract of Aspiliaafricana. .J. Ethnopharm. 109(2): 219-225.
Rajagopal S, Kumar RA, Deevi DS, Satyanarayana C, Rajagopalan R 2003. Andrographolide, a potential cancer therapeutic agent isolated from Andrographis paniculata. J Exp Ther Oncol 3:147–158.
Soundararajan Vijayarathna and Sasidharan Sreenivasan 2012. Antioxidant Activity of Elaeis guineensis Leaf Extract: An Alternative Nutraceutical Approach in Impeding Aging. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia.
Stanslas J, Liew PS, Iftikhar N, Lee CP, Saad S, Lajis Net al.2001. Potential of AG in the treatment of breast cancer. Eur J Cancer 37 (Suppl. 6): 169.
Sukardiman, H. S, Abdul Rahman, MuljaHadiSantosa and Firsa A. P. 2014. Ethyl acetate fraction of Andrographis paniculata (ness) increases cytotoxic effect of 5-fluorouracil on human cancer cell lines. International Journal of Pharmacy and Pharmaceutical Sciences. ISSN- 0975-1491 Vol 6; 5.
Talib W., Mahasneh A. 2010. Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scientia Pharmaceutica. 78:33-45
TaziebouLienou C., Etoa F-X., Nkegoum B., Pieme C. A. and Dzeufiet D.P.D 2007. Acute And Sub-acute Toxicityof Aspilia africana leaves. Afr. J.Trad. CAM 4 (2):127 – 134.
Thiyagarajan P., Deepak H. B. and Agarwal, A. (2011). In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract Chandrasekaran. International Immunopharmacology 11:1 (79-84).
Uduak E. and Ikoedem.U. 2013. Phytochemical and Physico-chemical Properties of Aspilia africana (Pers) C. D. Adams and Tithonia diversifolia (Hemsl) A. Gray Petals as a Scientific Backing to Their Tradomedicinal Potentials. International Journal of Modern Biology and Medicine, 3(2): 88-100.
Valerio L. G.,Jr and Gonzales G.F 2005.Toxicological aspects of the South American herbs cat's claw (Uncariatomentosa) and Maca (Lepidiummeyenii): a critical synopsis. Toxicol Rev. 2005; 24(1):11-35.
Wamidh H. Taliband Adel M. Mahasneh 2010.Antimicrobial, Cytotoxicity and Phytochemical Screening of Jordanian Plants Used in Traditional Medicine. Department of Biological Sciences, Faculty of Science, University of Jordan, Amman-11942, 15(3), 1811-1824
Wilasrusmee, C.; Kittur, S.; Siddiqui, J.; Bruch, D .; Wilasrusmee, S; Kittur, D. 2002 In vitro immunomodulatory effects of ten commonly used herbs on murine lymphocytes. J. Alternative Compl. Med., .8(4), p.467-475.
Zhang S, Zhou J, Ong CN, Shen HM 2006. Critical role of pro-apoptotic Bcl-2 family members in andrographolide-induced apoptosis in human cancer cells. J. Biochem. Pharmacol., 72: 132-144
Published
2018-05-02
Section
Section B (Basic Science Research)