Attenuation of Delayed Gastric Ulcer in Rats by Chrysophyllum Albidum Bark Extract and Fractions: Role of Blood Inflammatory Markers


  • AD Famurewa
  • Adeola T Salami


Chrysophyllum albidum has been reported to exert varied medicinal properties.  However information is lacking on its probable activities during chronic ulcer healing which this study sought to investigate. 120 male wistar rats (120-150g) were divided into 8 groups (n=15) viz: Groups I -delayed ulcerated untreated; II and III – 500 and 250mg/kg b.w methanolic bark extract of C. albidum (MeCaB) respectively; II, III and IV-100mg/kg b.w chromatographic fractions A, B and C (CFrA, B and C) respectively; VII –30mg/mg b.w Omeprazole and VIII-ulcerated untreated not-delayed(baseline group). Chronic gastric ulcer was induced experimentally and delayed using indomethacin; drug treatments occured simultaneously for 14 days. Daily body weights of experimental animals were monitored; hematological variables; ulcer score, and histological analysis were evaluated by days 3, 7 and 14 of treatment. Data were expressed as Mean±SEM, analyzed using one-way ANOVA and p£0.05 was considered statistically significant. The MeCaB, CFr A, B and C significantly increased body weights and haematological variable except (White blood cells) compared with untreated groups by days 3 and 7. The MeCaB and fractions significantly reduced Neutrophil/lymphocyte and Platelet/lymphocyte ratios (blood inflammatory makers) in this study. Furthermore, MeCaB (250 and 500mg/kg b.w) showed complete ulcer healing by day 3 with CFrA, B and C by day 7 of treatment. These observations were buttressed by histological evaluations. Chrysophyllum  albidum bark extracts and its chromatographic fractions enhanced ulcer healing by increasing nitric oxide levels and heamatological variables to gastric tissue. Blood inflammatory makers were also ameliorated to a low level


Adewoye, E.O., Salami, A.T., Emikpe, B.O (2012). Effect of the Methanolic Extract of Chrysophyllum albidum bark on hematological indices in mice with experimental hemorrhagic anaemia. Afr J Biomed Res 15: 85-91

Adewoye, E.O., Salami, A.T., Taiwo, V.O (2010). Antiplasmodial and Toxicological effects of methanolic bark extract of Chrysophyllum albidum in albino mice. Journal of Physiology and Pathophysiology, 1: 1-9

Adisa, S.A (2000). Vitamin C, Protein and mineral contents of African Apple (Chrysophyllum albidum) IN: Proceedings of the 18th annual conference of NIST. (Eds.) 141 -146

Ahbap, E., Sakaci, T., Kara, E., Sahutoglu, T., Koc, Y., Basturk, T., Sevinc, M., Akgol, C., Kayalar, A.O., Ucar Z.A., Bayraktar, F., Unsal, A. (2016). Neutrophil to lymphocyte ratio and platelet to lymphocyte ratio in evaluation of inflammation in end- stage renal disease. Clin Nephrol. 85 (4): 199-208.

Amure, B.O., Ginsburg, M (1964). Inhibitors of histamine catabolism and the action of gastrin in the rat. Br J Pharmacol Chemother. 23: 476-485

Asako, H., Kubes, P., Wallace, J.L., Gaginella, T., Wolf, R.E., Granger, D.N (1992b). Indomethacin induced leukocyte adhesion in mesenteric venules: Role of lipoxygenase products. Am J Physiol 262: G903-8

Alexander, N.I (2016). Reference values of neutrophil/lymphocyte ratio, platelet/lymphocyte ratio and mean platelet volume in healthy adults in North Central Nigeria. Journal of Blood and Lymph 6: 1

Brzozowska, I., Targosz, A., Sliwowski, Z., et al. (2004). Healing of chronic gastric ulcer in diabetic rats treated with native aspirin, nitric oxide derivative of aspirin and cyclooxygenase (COX)-2 inhibitors. J Physiol Pharmacol., 55: 773-90

Brzozowska, I., Knoturek, P.C., Brzozowski, .T, et al. (2002). Role of prostaglandin, nitric oxide, sensory nerves and gastrin in acceleration of ulcer healing by melatonin and its’ precursors, L-tryptophan. J Pineal Res 32: 149-62

Bada, S.O (1997). Preliminary information on the ecology of Chrysophyllum albidum done in the West and Central Africa; In proceedings of a National Workshop on the potentials of Star Apple in Nigeria, 16 – 25

Dacie, J.V., Lewis, S.M (1994). In: Practical Haematology 7th edition ELBS with Churchill Livingstone, Long man group Uk 5-82: 160-175

Gana, T.J., MacPherson, B.R., Koo, J (1988). Gastric mucosal blood flow in misoprostol pretreated aspirin-induced ulceration. Ann Surg. 207: 327-334

Guide for the Care and Use of Laboratory Animals, 8th ed., 2011, https:// pdf.

Idowu, T.O., Ogundaini, A.O., Adesanya, S.A., Onawunmi, G.O., Osungunna, M.O., Obuotor, E.M., and Abegaz B.M (2016). Isolation and characterization of chemical constituents from Chrysophyllum albidum G. Don-holl. stem-bark extracts and their antioxidant and antibacterial properties. Afr J Tradit Complement Altern Med. 13(5): 182–189

Ito, M., Sagami, T., Tsukahara, T., et al. (1994). Effect of cimetidine and omeprazole on gastric ulcer healing of rats with lSalami, A.T., Jonah, D.C., Chukwudi, I.M., Attah, F.A., Adeyemi, A.A., Moody, J.O., Silva, O.D (2015) In-vitro Antihelmintic and Kill Kinetics Activities of Stem Bark Extracts and Chromatographic Fractions of Chrysophyllum albidum (G. don). Arch Bas App Med., 3: 29-36

Jing, C., Dongsheng, H., You, Z., Peng, S (2015). Meta-analysis of association between neutrophil to lymphocyte ratio and prognosis of gastric cancer. World Journal of Surgical Oncology, 13: 122

Khalid, G.A., Ali, J.A. (2014). The protective role of Camel’s milk on some haematological parameters of male rats infected with gastric ulcer. World Journal of Pharmaceutical Sciences. 29: 1465-1468.

Laine, L., Takeuchi, K., Tarnawski, A. (2008). Gastric mucosal defense and cytoprotection: bench to bedside. Gastroenterology, 135: 41-60.

Ma, L, McKnight, W., Dicay, M., Klein, A., Hollenberg, M.D., Wallace, J.L (2005). Proteinase-activated receptors 1 amd 4 counter-regulate endostatin and VEGF release from human platelets. Proc. Natl. Acad. Sci. U.S.A. 102: 216-220.

Maloney, J.P., Silliman, C.C., Ambruso, D.R., Wang, J., Tuder, R.M., Voelkel, N.F (1998) In vitro release of vascular endothelial growth factor during platelet aggregation. Am J Physiol. 275: H1054-H1061

Marcus, C., Karin, L., Jain, G., Matthias, L., Jorns, F., Tilman, G., Jurgen, W.S (2003). Captive roe deer (Capreolus capreolus) select for low amount of tannic acid but not quebracho: fluctuation of preference and potential benefit. Com. Biochem and Physio. Part B: Biochem and Molec. Biolo. 136: 369-382

Mota, K.S., Dias, G.E., Pinto, M.E. et al. (2009). Flavonoids with gastroprotective activity. Molecules 14: 979-1012

Okabe, S., Kikuko, A., Koji, T. (2010). Acetic acid ulcer model-state of the art in 2010. Gastroentelogia Polska 17: 165-168

Olsen, S.P., Therkelsen, K., Poulsen, S.S (1988). Effect of omeprazole and cimetidine on healing of chronic gastric ulcers and gastric acid secretions in rats. Tohoku J Exp Med. 155: 305-310

Palmer, E.D (1973). Benign chronic gastric ulcer and weight loss. Am Fam Physician 8: 109

Salami, A.T., Odukanmi, A.O., Olagoke, C.O., Iyiola, T.O., Olaleye, S.B (2016). Role of nitric oxide and endogenous antioxidants in thyroxine facilitated healing of ischemia-reperfusion induced gastric ulcers. Nig J Pharm Res. 12: 189-206

Salami, A.T., Oyelami, AI., Omayone, T.P., Adewoye E.O., Olaleye, S.B. (2014). Effect of methanolic bark extracts of Chrysophyllum albidum on acetic acid induced colitis in rats. Proceedings of the 9th congress of European Crohn’s and Colitis organization.

Salami, A.T., Odukanmi, A.O., Oshode, O.O. Olaleye, S.B (2017). Modulatory activities of Chrysophyllum albidum and its fractions on microflora and colonic pump activities during inflammatory phase of colitis healing in experimental mice. Food Bioscience,

Salami, A.T, Adeola B.O, Iyiola T.O, Omayone, T.P, Oluwole F.S., Olaleye S.B (2015). Antioxidative action of Manganese treatment in delayed healing of acetic acid induced ulceration in rat stomach. Journal of African Association of Physiological Sciences Vol. 3. (2): 67-78

Schmassmann A, Tarnawski A, Peskar BM, Varga L, Flogerzi B., Halter F (1995). Influence of acid and angiogenesis on kinetics of gastric ulcer healing in rats: interaction with indomethacin. Am. J. Physiol 268: G276-G285.

31 Shimuzi N, Wantabe T, Arakawa T, Fujiwara Y, Higuchi K, Kuroki T (2000). Pentoxifylline accelerates gastric ulcer healing in rats: roles of tumour necrosis factor and neutrophils during the early phase of ulcer healing. Digestion 6: 157-164

Suzuki, H., Inadomi, J.M., Hibi, T. (2009). Japanese herbal medicine in functional gastrointestinal disorders. Neurogastroenterology and motility, 21: 688-696

Szelenyi, I., Engler, H., Hwrzog, P., Postius, S., Vergin, H., Holtermuller, K (1982). Influence of non-steroidal anti-inflammatory compounds on healing of chronic gastric ulcers in rats. Agents and Actions 12: 180-182

Tulassay, Z., Herszenyi, L. (2010). Gastric mucosal defense and cytoprotection. Best Pract Res Clin Gastroenterol 24: 99-108

Turken, K (2013). Platelet to lymphocyte ratio: one of the novel and valuable platelet indices in hemodialysis patients. Hemodial Int. 17: 670

Wallace, J.L., Dicay, M., McKnight, W. and Dudar, G.K. (2006). Platelets accelerates gastric ulcer healing through presentation of vascular endothelial growth factor. British Journal of Pharmacology 148: 274-278

Watson, J., Round, A. Hamilton, W (2012). Raised Inflammatory markers. BMJ. 344: e454





Pathology and System Biology